The Ward lab at UC Santa Cruz
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    • pha-1 co-conversion >
      • Strain maintenance
      • cku-80 RNAi
      • Construct and oligo design
      • Generation of PU6::sgRNA templates by PCR fusion
      • Injections
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Molecular Biology

Picture
  • 10cm and 15 cm plate grids for colony PCR
  • Colony PCR protocol (using Phusion polymerase)
  • Colony PCR protocol (using homemade Taq/Pfu polymerase)
  • Electrocompetent E. coli preparation
  • Ethanol precipitation of DNA
  • Gateway cloning pipeline
  • PEG/DMSO competent cells
  • qPCR (using BioRad Sso polymerase)
  • qPCR primer validation
  • RNA purification
  • Yeast colony PCR


Methods and protocols overview
Biochemistry
C. elegans
pha-1 co-conversion
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  • Home
  • Research
    • Molting
    • Spermatogenesis
    • Tool development
  • People
    • Current Ward Lab Members
    • Former lab members
    • Positions
  • Publications
    • Peer Reviewed Publications
    • Preprints
    • K99 grant writing
    • Our neglected blog
    • Meeting slides >
      • 2017 Worm meeting
      • 2019 Worm meeting
  • Methods & protocols
    • Biochemistry
    • C. elegans
    • Molecular biology
    • pha-1 co-conversion >
      • Strain maintenance
      • cku-80 RNAi
      • Construct and oligo design
      • Generation of PU6::sgRNA templates by PCR fusion
      • Injections
      • FAQs
  • Contact us