The Ward lab at UC Santa Cruz
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Preprints

​We believe in disseminating research products of all kinds. We pre-print all work before publication and we highlight pre-printed work that is under review, when appropriate.
Conditional depletion reveals temporal requirements for the oscillating transcription factor NHR-23/NR1F1 in C. elegans larval progression
Johnson LC,  Aguilera J,  Levenson MT,  Rechtsteiner A,  Vo AA,  Ragle JM,  Ward JD. bioRxiv 2021. doi: https://doi.org/10.1101/2021.10.27.465992

An engineered, orthogonal auxin analog/AtTIR1(F79G) pairing improves both specificity and efficacy of the auxin degradation system in Caenorhabditis elegans
Kelly Hills-Muckey, Michael A. Q. Martinez, Natalia Stec, Shilpa Hebbar, Joanne Saldanha, Taylor N. Medwig-Kinney, Frances E. Q. Moore, Mariia Ivanova, Ana Morao, Jordan D. Ward, Eric G. Moss, Sevinc Ercan, Anna Y. Zinovyeva, David Q. Matus, Christopher M. Hammell
bioRxiv 2021. doi: https://doi.org/10.1101/2021.08.06.455414

The conserved molting/circadian rhythm regulator NHR-23/NR1F1 serves as an essential co-regulator of C. elegans spermatogenesis
James Matthew Ragle, Abigail L. Aita, Kayleigh N. Morrison, Raquel Martinez-Mendez, Hannah N. Saeger, Guinevere A. Ashley, Londen C. Johnson, Katherine A. Schubert, Diane C. Shakes, Jordan D. Ward
bioRxiv 2020. doi: https://doi.org/10.1101/2020.06.11.147298

Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing
Guinevere Ashley, Tam Duong, Max T. Levenson, Michael A. Q. Martinez, Jonathan D. Hibshman, Hannah N. Saeger, Ryan Doonan, Nicholas J. Palmisano, Raquel Martinez-Mendez, Brittany Davidson, Wan Zhang, James Matthew Ragle, Taylor N. Medwig-Kinney, Sydney S. Sirota, Bob Goldstein, David Q. Matus, Daniel J. Dickinson, David J. Reiner, Jordan D. Ward
bioRxiv 2020. doi: https://doi.org/10.1101/2020.05.12.090217

Rapid degradation of C. elegans proteins at single-cell resolution with a synthetic auxin
Michael A. Q. Martinez, Brian A. Kinney, Taylor N. Medwig-Kinney, Guinevere Ashley, James M. Ragle, Londen Johnson, Joseph Aguilera, Christopher M. Hammell, Jordan D. Ward, David Q. Matus
bioRxiv 2019. doi: https://doi.org/10.1101/716837

The glucocorticoid receptor ligand-binding domain confers drug-gated protein regulation in C. elegans
Gabriela C. Monsalve, Keith R. Yamamoto, Jordan D. Ward
bioRxiv 2018. 445833; doi: https://doi.org/10.1101/445833
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  • Home
  • Research
    • Molting
    • Spermatogenesis
    • Tool development
  • People
    • Current Ward Lab Members
    • Former lab members
    • Positions
  • Publications
    • Peer Reviewed Publications
    • Preprints
    • K99 grant writing
    • Our neglected blog
    • Meeting slides >
      • 2017 Worm meeting
      • 2019 Worm meeting
  • Methods & protocols
    • Biochemistry
    • C. elegans
    • Molecular biology
    • pha-1 co-conversion >
      • Strain maintenance
      • cku-80 RNAi
      • Construct and oligo design
      • Generation of PU6::sgRNA templates by PCR fusion
      • Injections
      • FAQs
  • Contact us